Microtubule-associated protein MAP1 promotes microtubule assembly in vitro
نویسندگان
چکیده
منابع مشابه
Cep70 promotes microtubule assembly in vitro by increasing microtubule elongation.
Microtubules are dynamic cytoskeletal polymers present in all eukaryotic cells. In animal cells, they are organized by the centrosome, the major microtubule-organizing center. Many centrosomal proteins act coordinately to modulate microtubule assembly and organization. Our previous work has shown that Cep70, a novel centrosomal protein regulates microtubule assembly and organization in mammalia...
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Objective(s):EMAP-like Protein 5 (EML5) is a new echinoderm microtubule-associated protein that is expressed in the central nervous system. The aim of this study was to investigate the expression profile of EML5 in the anterior temporal neocortex of patients presenting with intractable epilepsy (IE). Materials and Methods:Western blot assays were performed to determine EML5 expression in 36 su...
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Chicks were injected intra-cerebrally with [(3)H]leucine, and (3)H-labeled microtubules were assembled in vitro in the brain supernatants. Pieces of these (3)H-labeled microtubules were then incubated with unlabeled brain tubulin subunits under conditions where the subunits assembled onto the labeled pieces. Electron microscopic autoradiography of the negatively-stained microtubules showed all ...
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Microtubule-associated proteins (MAPs) promote the assembly of microtubules from purified tubulin in vitro. In order to establish a model system for the investigation of the role of MAPs in microtubule assembly in vivo, we have generated Saccharomyces cerevisiae strains that permit the modulation of the expression levels of MHP1 (MAP-Homologous Protein 1) and of the alpha and beta-tubulin genes...
متن کاملMicrotubule-associated protein 1B
Myelin-associated glycoprotein (MAG) is expressed in periaxonal membranes of myelinating glia where it is believed to function in glia-axon interactions by binding to a component of the axolemma. Experiments involving Western blot overlay and coimmunoprecipitation demonstrated that MAG binds to a phosphorylated neuronal isoform of microtubule-associated protein 1B (MAP1B) expressed in dorsal ro...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1981
ISSN: 0014-5793
DOI: 10.1016/0014-5793(81)80791-0